Objective To observe the effect of virtual reality technique in combination with comprehensive rehabilitation and recuperation therapy on the functional recovery of upper limb in the patients of upper extremity dysfunction after stroke. Methods Forty patients with upper extremity dysfunction after stroke admitted in our sanatorium from January to December 2015 were recruited in this study. They were randomly divided into the treatment group (n=20, routine rehabilitation training and virtual reality technique combined with comprehensive rehabilitation and recuperation) and the control group (n=20, only routine rehabilitation training). Their motor function (Fugl-Meyer score), activity of daily living (modified Barthel index) and muscle strength of the affected upper limb were observed before and in 10 weeks after treatment, and the results were compared between the 2 groups. Results The Fugl-Meyer score, modified Barthel index and muscle strength score were significantly higher after the treatment than before in the both groups of patients (P<0.05). In 10 weeks after treatment, the treatment group had obviously higher scores of above indices than the control group (P<0.05). Conclusion Virtual reality technique combined with comprehensive rehabilitation and recuperation significantly improves the motor function, activity of daily living and muscle strength of upper limb in the patients with upper extremity dysfunction after stroke. It is worthy of promotion in clinical practice.