Objective To determine the expression and significance of phosphatase and tensin homologue deleted on chromosome 10 (PTEN) in the marginal area of myocardial infarction, and the effect of peroxisome proliferator-activated receptor gamma agonist (PPAR-γ), rosiglitazone, on the expression in acute myocardial infarction (MI) rabbit model after the direct current (DC) electric fields (EFs) stimulation. Methods Fifty rabbits were randomly divided into sham-operation group (SH group, n＝5), MI group (n＝15), DCEFs treatment group (EFs group, n＝15), and DCEFs+rosiglitazone treatment group (R-EFs group, rosiglitazone 4 mg/kg·d, i.g.). After the corresponding model was established by occluding left anterior descending branch in the later 3 groups, DCEFs stimulation (4.0V/cm, 30min/d) was given to the later 2 groups for 1, 2 and 4 weeks respectively (n＝5 for each time point). The expression of PTEN in the ischemia myocardium was detected by Western blotting. Meanwhile, the ratio of left or right ventricular weight/body mass (LVW/BS or RVW/BS) and the hemodynamic indexes were measured. Results There was some expression of PTEN in cardiac tissue of SH group and MI group. The expression of PTEN in EFs group was higher than in MI group(P＜0.01), and the expression in R-EFs group was higher than EFs group(P＜0.05). In the meantime, compared to MI group, left ventricular function was improved in EFs group and R-EFs group. Conclusion PPARγ promotes the expression of PTEN and thus further improves the left ventricular systolic function.