Objective To observe the clinical outcome of ozone autologous blood transfusion therapy combined with vacuum-assisted closure(VAC) therapy in the treatment of diabetic foot ulcer. Methods A total of 28 patients with diabetic foot ulcer admitted in Heilongjiang Provincial Hospital from August 2014 to August 2015 were enrolled in this study. They were all treated by above-mentioned therapy. Transcutaneous oxygen partial pressure (TcPO2), total cholesterol (TC), triglycerides (TG), thromboxane B2 (TXB2), and 6-keto-prostaglandin F1α (6-k-PGF1α) were measured before and after treatment. Results The levels of TG, TC and TXB2 were declined, while that of TcPO2 and PGF1α was increased after the treatment when compared with before treatment (P＜0.01) . Conclusion Above-mentioned therapy achieves significant outcome in treatment of diabetic foot ulcer, and is worth further investigation.