Objective To explore the relationships between restless legs syndrome (RLS) and the motor symptoms or non-motor symptoms (NMS) in patients with Parkinson’s disease (PD). Methods Totally 165 PD patients were collected and divided into RLS group and non-RLS (NRLS) group according to the scores of RLS-RS and a comparison study was conducted by using related motor symptoms and NMS scales. Results (1) 42.4% of PD patients were accompanied by RLS. RLS group had significantly longer disease duration than NRLS group (P＜0.05). (2) There were significant differences in Hoehn-Yahr Stage and UPDRS Ⅲ score between RLS and NRLS groups (P＜0.05). (3) There were significant differences in the incidences of wearing-off and dyskinesia between the two groups (P＜0.05); There was no significant difference in the incidence of “on-off” phenomenon between the two groups (P＞0.05). (4) The numbers of NMS in RLS group was significantly more than that in NRLS group (P＜0.05). The incidences of recent memory loss, depression, anxiety, distraction, apathy and sexual dysfunction in RLS group were significantly higher than that in NRLS group (P＜0.05). (5) The scores of UPDRS Ⅰ, HAMD, HAMA, PSQI, FS-14, FSS and SCOPA-AUT in RLS group were significantly increased compared with NRLS group (P＜0.05). The scores of MoCA, MAES, ESS and RBD were not significantly different between the two groups (P＞0.05). (6) The scores of UPDRS Ⅱ and PDQL were significantly different between the two groups (P＜0.05). Conclusion RLS is one of the common NMS in PD, which is associated with their severity of motor symptoms and movement complications. PD patients with RLS have more NMS and show the high incidences of some NMS. PD patients with RLS present severer disturbances in spirit, emotion, automatic function and sleep, which dramatically compromise the activities of daily living and quality of life.