Objective To determine therapeutic effect of Xuebijing (XBJ) injection, a compound injection of traditional Chinese herbs, on multiple organ dysfunction syndrome at high altitude (H-MODS). Methods A total of 18 sheep were randomly divided into control, model and XBJ treatment groups, with 6 sheep in each group. After the sheep quickly reached a highland of 3500m, H-MODS model was inflicted by venous injection of 6μg/kg lipopolysaccharide (LPS), and LPS was substituted with normal saline in the same dose for control group. After that, the sheep in model and XBJ groups were given an intravenous injection of 2ml/kg normal saline and 2ml/kg XBJ injection respectively, twice per day for 3 consecutive days. Biochemical parameters and blood routine indices were detected in the blood samples collected at 0, 3, 12, 24, 48 and 72h after model establishment. Results The contents of creatine kinase (CK), creatine kinase isoenzyme (CK-MB), aspartic transaminase (AST), alanine aminotransferase (ALT), total bilirubin (TBIL) and creatinine (Cr) were significantly higher in the model group than in the control and treatment groups (P＜0.05 or P＜0.01) at the different time points. Compared with the control, the number of white blood cells (WBC) was obviously decreased rapidly in the model and treatment groups at 3h, and then began to elevate suddenly in another 3h later. But the increasing tendency was more smoothly in the treatment group than in the model group. Conclusion XBJ injection exerts protective effect on the heart, lung, liver, and kidney in sheep against H-MODS injury.