Objective To evaluate the relationship between aging-related cardiovascular subclinical state and estimated glomerular filtration rate (GFR) in healthy population in Shenyang. Methods In the cross-sectional study, 505 healthy people in Shenyang area were devided into ≤60 years and ＞60 years group. The physical condition, blood pressure, blood biochemical examination and cardiovascular ultrasound were performed. GFR was evaluated by Cockcroft-Gault equation (GFRCG), Chinese modified MDRD formula (GFRMDRD), Chronic Kidney Disease Epidemiology Collaboration equation (GFRCKD-EPI). Cardiac systolic function was assessed by the left ventricular ejection fraction (LVEF). Cardiac diastolic function was assessed by the ratio between mitral E/A ratio, deceleration time of the early mitral velocity (MV-DT), left atrial volume index(LAVI). Vascular structure and stiffness were assessed by carotid intema-media thickness (IMT), ankle brachial index (ABI) and pulse wave velocity (PWV). Results In ≤60 years group, GFRCG was significantly negative correlated with IMT (r＝-0.238, P＜0.01) and PWV (r＝-0.281, P＜0.01). There was significant negative correlation between GFRMDRD and LVEF (r＝-0.221, P＜0.01). GFRCKD-EPI was significantly negatively correlated with IMT (r＝-0.360, P＜0.01) and PWV (r＝-0.327, P＜0.01). After adjusting for age, body mass index (BMI), waist-hip ratio (WHR), blood pressure, blood lipid, and GFRCG was significantly positively correlated with LAVI (r＝0.168, P＜0.05). There was significant negative correlation between GFRMDRD and LVEF (r＝-0.212, P＜0.01). A significant positive correlation of GFRCKD-EPI with LAVI (r＝0.195, P＜0.01), and negative correlation with EA (r＝-0.137, P＜0.05) were observed. In ＞60 years group, GFRMDRD was positively correlated with ABI (r＝-0.167, P＜0.05), which remained significant (r＝0.175, P＜0.05) even after adjusting for confounding variables. Conclusion In aging process, there is decline in renal function, arterial stiffness and cardiac diastolic function. Our results confirm that there is interaction of cardiovascular system and renal function even in healthy people.