Objective To investigate the effect of gastrointestinal administration of hemin on oxidative stress in rat model of heart failure induced by pressure-overload. Methods Sixty-three adult male Sprague-Dawley rats were divided into control group (group C),heart failure group (HF group) and hemin group (Hm group) randomly,with 21 in each group. Each group was further divided into three subgroups (n=7) for the time of 4 weeks,8 weeks and 12 weeks after heart failure established. The rats in HF and Hm groups were subjected to surgical abdominal aorta constriction,while rats in control group to sham operation. At 3 weeks after surgery,rats in Hm group received daily gastric administration of hemin (60 mg/kg),while rats in HF and control groups received equal volume of normal saline. At each time points,the plasma levels of heme oxygenase-1 (HO-1),carboxyhemoglobin(COHb),malondialdehyde (MDA),oxidized low density lipoprotein (ox-LDL),and the activity of superoxide dismutase(SOD) were detected. Results (1) Compared with HF group,the plasma levels of HO-1 and COHb in Hm group were obviously increased at each time points HO-1: (6.80±0.92) μg/L vs (2.50±0.22) μg/L;(10.70±0.69) μg/L vs (4.50±0.28) μg/L;and (13.30±0.99) μg/L vs (6.07±0.71) μg/L,P<0.01; COHb: (4.34±0.31)% vs (1.68±0.11)%;(6.32±0.44)% vs (2.46±0.20) %;and (7.80±0.39)% vs (3.01±0.42)%,P<0.01. (2) Compared with HF group,the MDA levels were obviously decreased in Hm group at each time points (8.3±1.3) μmol/L vs (14.2±2.3) μmol/L,P<0.05;(9.6±0.5) μmol/L vs (20.2±3.2) μmol/L,P<0.01;and (11.8±1.1) μmol/L vs (26.0±3.7) μmol/L,P<0.01. (3) SOD activity was higher in Hm group than in HF group at each time points (125±6) kU/L vs (95±10) kU/L,P<0.01;(109±9) kU/L vs (67±5) kU/L,P<0.01;and (72±10) kU/L vs (40±6) kU/L,P<0.01. (4)The content of ox-LDL was lower in Hm group than in HF group at each time points (1.14±0.16) μmol/L vs (1.80±0.22) μmol/L,(1.38±0.14) μmol/L vs (2.34±0.25) μmol/L,and (1.83±0.16) μmol/L vs (3.17±0.31) μmol/L,P<0.01. Conclusion Production of HO-1 can be induced by gastrointestinal administration of hemin. The induction of HO-1/CO-bilirubin system may correct the in vivo unbalance of oxidation/antioxidation in HF rat induced by pressure-overload.?更多