Objective To study the influence of experimental acute pulmonary thromboembolism (PTE) on hepatocyte growth factor (HGF) levels in plasma and tissue of rabbit,and to explore the diagnostic value of HGF in PTE. Methods Thirty-two rabbits were randomly used for detection of HGF in plasma (n=16) and tissues (n=16). For each detection,the rabbits were further divided into control group and PTE model group,with 8 in each. The PTE model was established by intravenous injection of autologous blood clots,and the control group was injected with isometric normal saline. The HGF (0,1,3,6,12,24,48,72 h) level in plasma was detected by ELISA. The rabbits were sacrificed at 12 h,and the expression of HGF in tissue of lung,liver,and right side of the heart was detected by immunohistochemistry. Results The plasma HGF level was significantly higher in PTE group than in control group. It began to elevate at 1h after PTE,and reached its peak at 12 h,significantly higher than that in control group〔(1.74±0.49) vs (0.26±0.23) μg/L,P<0.001〕. Then,it began to reduce gradually at 72 h. However,it remained at low level in control group through the whole experimental duration (P<0.5 μg/L). In tissue of lung and right side of the heart,the expression of HGF was significantly higher in PTE group than in control group,while in liver tissue,it was not significantly different between PTE group and control group. The HGF expression in lung tissue was significantly elevated compared with right side of the heart and liver tissues. Conclusion HGF can be used for the adjunctive diagnosis of PTE.?更多