Objective To determine the influence of recombinant human relaxin (rhRLX) on collagen gene expression in cardiac fibroblasts (CFs) under high glucose (HG) conditions. Methods CFs were isolated from Sprague-Dawley rats and cultured primarily. The mRNA levels of type Ⅰ,type Ⅲ collagen and RLX in CFs after 3 passages under normal glucose (NG,5.6 mmol/L) and high glucose (HG,25 mmol/L) conditions were assessed by RT-PCR. rhRLX (100 μg/L) was cocultured with CFs under NG and HG conditions for 72 h respectively,then the mRNA levels of type Ⅰand type Ⅲ collagen were detected. Results HG increased the expression of type Ⅰ and type Ⅲ collagen,which was inhibited by rhRLX. The RLX mRNA expression was stronger in HG group than in NG group. Conclusion rhRLX inhibits the HG-enhanced overexpression of typeⅠand type Ⅲ collagen.?更多