Effect of telmisartan on mature adipocytes investigated with microarray based high throughput gene expression profile
  
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Key words:angiotensinⅡreceptor blocker  gene expression microarray analysis  adipocyte  
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CHEN Kang, et al  
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Abstract:
      Objective To screen the differentially expressed genes related to lipid metabolisms in the mature adipocytes and to investigate the effect of telmisartan on the cells with microarray based high throughput gene expression profile.Methods The 3T3-L1 preadipocytes were induced into the mature adipocytes, which was then intervened with telmisartan (0.1mg/L). The total RNA was isolated from the cells with or without telmisartan intervention with Trizol method. After purification, reverse transcription was carried out to synthesize Cy3/Cy5 labeled cDNA probe. The probe was hybridized with microarray based high throughput gene expression profile (36 000 genes or gene fragments) to screen the differentially expressed genes in the cells with and without telmisartan intervention. The effect of telmisartan on the function of the mature adipocytes and the possible signal pathway were analyzed.Results A total of 157 differentially expressed genes were screened out, including 86 up-regulated genes and 71 down-regulated ones, which were involved in lipid synthesis, catabolism and transport in the adipocytes. The changes in the expression level of these genes might be related to the effects of telmisartan on function of mature adipocytes, such as cytokine-cytokine receptor interaction, cell adhesion molecules, adipocytokine signaling pathway, oxidative stress, etc. In addition, telmisartan played some roles in certain pathways related to the proliferation, differentiation, and lipid synthesis of mature adipocytes, such as Wnt, β-catenin, and Notch signaling pathways.Conclusion Telmisartan exerts its effect on mature adipocytes in a manner which is independent of AT receptor. Telmisartan may affect the lipid synthesis, catabolism and transport of the mature adipocutes.
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