Objective To observe the effectiveness of N-terminal pro-brain natriuretic peptide (NT-proBNP), estimated glomerular filtration rate (eGFR) and high sensitivity C-reactive protein (hs-CRP) in risk stratification for the development of type 1 cardiorenal syndrome (CRS1) in patients with acute myocardial infarction (AMI). Methods A retrospective analysis was made of the available data of NT-proBNP, eGFR, and hs-CRP for 2 094 AMI patients in Cardiovascular Center of Beijing Friendship Hospital Database Bank (CBD BANK). The patients were divided into quartiles of an approximately equal number to assess the relationship of the three biomarkers with CRS1 incidence and in-hospital mortality. Statistical analyses were performed using SPSS 22.0. Kaplan-Meier survival analysis and COX proportional hazard regression were used to assess the effect of the number of abnormal biomarkers on in-hospital mortality. Results The incidence of CRS1 and in-hospital mortality increased in AMI patients as NT-proBNP and hs-CRP increased, and eGFR decreased (P<0.05). As CRS1 was aggravated, eGFR tended to decrease, and NT-proBNP and hs-CRP tended to increase before CRS1 became severe. Kaplan-Meier survival analysis showed that the in-hospital mortality in the AMI patients was correlated with the number of abnormal biomarkers (P<0.001). Abnormal biomarkers ≥2 was an independent risk factor for in-hospital death (RR＝2.452, 95%CI 1.105-5.440; P=0.027). Conclusion The combination of NT-proBNP, eGFR, and hs-CRP at presentation may assist risk stratification for the development of CRS1 in AMI patients.