|
Effect of bisdemethoxycurcumin on staurosporine-induced apoptosis in cardiomyocytes |
Received:July 06, 2016 Revised:August 18, 2016 |
View Full Text View/Add Comment Download reader |
DOI£º10.11915/j.issn.1671-5403.2017.01.013 |
Key words:bisdemethoxycurcumin staurosporine apoptosis cardiomyocytes |
Author Name | Affiliation | E-mail | LI Xing | Department of Geriatrics, Xijing Hospital, the Fourth Military Medical University, Xi¡¯an 710032, China | xmwang@fmmu.edu.cneffect | HUO Cong | Department of Geriatrics, Xijing Hospital, the Fourth Military Medical University, Xi¡¯an 710032, China | xmwang@fmmu.edu.cneffect | LIU Yan | Department of Geriatrics, Xijing Hospital, the Fourth Military Medical University, Xi¡¯an 710032, China | xmwang@fmmu.edu.cneffect | XU Rong | Department of Geriatrics, Xijing Hospital, the Fourth Military Medical University, Xi¡¯an 710032, China | xmwang@fmmu.edu.cneffect | XIA Yue-Sheng | Department of Geriatrics, Xijing Hospital, the Fourth Military Medical University, Xi¡¯an 710032, China | xmwang@fmmu.edu.cneffect | JIA Xin | Department of Geriatrics, Xijing Hospital, the Fourth Military Medical University, Xi¡¯an 710032, China | xmwang@fmmu.edu.cneffect | WANG Xiao-Ming | Department of Geriatrics, Xijing Hospital, the Fourth Military Medical University, Xi¡¯an 710032, China | xmwang@fmmu.edu.cneffect |
|
Hits: 1552 |
Download times: 958 |
Abstract: |
Objective To determine the effect of bisdemethoxycurcumin (BDMC) on the apoptosis of primary cardiomyocytes induced by staurosporine (STS). Methods Myocardial cells were isolated from neonatal C57BL/6J mice and then primarily cultured. Then the obtained cells were divided into control group, STS treatment group, BDMC pretreatment+STS treatment group and BDMC pretreatment group. Cell viability, cell apoptosis, caspase-3 activity, and intracellular reactive oxygen species (ROS) level were measured by CCK-8 assay, TUNEL assay kit, caspase-3 activity assay kit and ROS assay kit, respectively. Results Pretreatment of 100 ¦Ìmol/L BDMC resulted in significantly increased cell viability, obviously decreased caspase-3 activity, remarkably lowered apoptosis rate and notably reduced ROS level (P<0.05) in the cardiomyocytes. Conclusion BDMC can inhibit cell apoptosis through decreasing the intracellular ROS level in the cardiomyocytes, so as to protect the cells against STS-induced injury. |
Close |
|
|
|