Effect of bisdemethoxycurcumin on staurosporine-induced apoptosis in cardiomyocytes
Received:July 06, 2016  Revised:August 18, 2016
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DOI£º10.11915/j.issn.1671-5403.2017.01.013
Key words:bisdemethoxycurcumin  staurosporine  apoptosis  cardiomyocytes
Author NameAffiliationE-mail
LI Xing Department of Geriatrics, Xijing Hospital, the Fourth Military Medical University, Xi¡¯an 710032, China xmwang@fmmu.edu.cneffect 
HUO Cong Department of Geriatrics, Xijing Hospital, the Fourth Military Medical University, Xi¡¯an 710032, China xmwang@fmmu.edu.cneffect 
LIU Yan Department of Geriatrics, Xijing Hospital, the Fourth Military Medical University, Xi¡¯an 710032, China xmwang@fmmu.edu.cneffect 
XU Rong Department of Geriatrics, Xijing Hospital, the Fourth Military Medical University, Xi¡¯an 710032, China xmwang@fmmu.edu.cneffect 
XIA Yue-Sheng Department of Geriatrics, Xijing Hospital, the Fourth Military Medical University, Xi¡¯an 710032, China xmwang@fmmu.edu.cneffect 
JIA Xin Department of Geriatrics, Xijing Hospital, the Fourth Military Medical University, Xi¡¯an 710032, China xmwang@fmmu.edu.cneffect 
WANG Xiao-Ming Department of Geriatrics, Xijing Hospital, the Fourth Military Medical University, Xi¡¯an 710032, China xmwang@fmmu.edu.cneffect 
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Abstract:
      Objective To determine the effect of bisdemethoxycurcumin (BDMC) on the apoptosis of primary cardiomyocytes induced by staurosporine (STS). Methods Myocardial cells were isolated from neonatal C57BL/6J mice and then primarily cultured. Then the obtained cells were divided into control group, STS treatment group, BDMC pretreatment+STS treatment group and BDMC pretreatment group. Cell viability, cell apoptosis, caspase-3 activity, and intracellular reactive oxygen species (ROS) level were measured by CCK-8 assay, TUNEL assay kit, caspase-3 activity assay kit and ROS assay kit, respectively. Results Pretreatment of 100 ¦Ìmol/L BDMC resulted in significantly increased cell viability, obviously decreased caspase-3 activity, remarkably lowered apoptosis rate and notably reduced ROS level (P<0.05) in the cardiomyocytes. Conclusion BDMC can inhibit cell apoptosis through decreasing the intracellular ROS level in the cardiomyocytes, so as to protect the cells against STS-induced injury.
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