Objective To investigate the relationship between polymorphism of IL-8+781 and risk of ovarian cancer. MethodsPatients with ovarian cancer and age-matched healthy people were enrolled in this study. The polymorphism of IL-8+781 was detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results The individuals with genotype TT had higher risk of ovarian cancer, compared with those with genotype CC (OR=3.385, 95%CI: 1.361-8.418, P=0.009). The individuals carrying allele T had higher risk of ovarian cancer than those carrying allele C (OR=1.704, 95%CI: 1.122-2.590, P=0.013). Conclusion IL-8+781 polymorphism may exert an effect on the occurrence of ovarian cancer.