Objective To investigate the prevalence, antibiotic resistance, virulence and pathogenicity of community-acquired Staphylococcus aureus (CA-SA) in the patients with skin soft-tissue infections. Methods Clinical specimen of the 55 patients with skin soft-tissue infections admitted in Department of Dermatology, Outpatient Department and Emergency Department in our hospital between January 2009 and August 2010 were collected. The isolates of SA strains were collected from the patients for bacterial identification and antibiotic susceptibility. Multilocus-sequence typing (MLST), staphylococcal protein A (SPA) typing and toxin gene screening were also performed. ResultsOf the 55 isolates, 12 strains of community-acquired methicillin-sensitive SA (CA-MSSA) were isolated. The CA-MSSA strains had comparatively high resistance rates (8.3%-50.0%) to erythromycin, tetracycline, clindamycin, gentamicin and levofloxacin, but were sensitive to other antibiotics. Their toxin positive rates were 33.3%, 25.0% and 8.3%, respectively to Panton-Valentine leukocidin (pvl), staphylococcal entotoxin C (sec) and toxic shock syndrome toxin-1 (tsst-1), but were none to staphylococcal entotoxin H (seh) and staphylococcal exfoliative toxin (et). The MLST and SPA typing results showed that there were 2 isolates of ST5-t002, 2 isolates of ST22-t309, and 1 isolate respectively of ST398-t034, ST15-t5864, ST7-t091, ST25-t078, ST30-t318, ST121-t1425, ST800-t1425 and ST630-t377. Conclusion The CA-MSSA isolates derived from the patients with skin soft-tissue infections of our hospital have high antibiotic sensitive rates, diversity of molecular types and multiple toxins.