Significance of pressure-volume loop in assessment of left ventricular function in pressure-overload heart failure rats
Received:May 05, 2016  Revised:June 27, 2016
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DOI:10.11915/j.issn.1671-5403.2016.08.0147
Key words:pressure-volume loop  pressure overload  heart failure  left ventricular function
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WANG Chen-Yang1, CAO Xue-Bin2*, XU Peng2, CAO Hai-Tao1, LIU Hai-Yan1 1Department of Animal Experiment, 2Department of Cardiology, Chinese PLA Hospital No. 252, Baoding 071000, China caoxb252@163.com 
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Abstract:
      Objective To determine the effect of chronic pressure-overload on the left ventricular hemodynamics in heart failure (HF) rats by pressure-volume (P-V) loop. Methods A total of 13 SD rats were randomly divided into sham operation (Sham) group (n=7) and HF group (n=6). The rat model of HF was induced by abdominal aorta coarctation, and the rats of the Sham group were inflicted with threading but not narrowing. After the animals were anesthetized, a Millar pressure volume conductance catheter (SPR-838) was inserted into left ventricule through right carotid artery to measure P-V loop. Results Compared with the Sham group, the heart mass (P<0.01) and the lung mass (P<0.05) were increased in the HF group (P<0.05). The steady-state P-V loop showed a notable elevation, rightward shift, and elevated slope of P-V curve in the HF group. Compared with the Sham group, the left ventricular end-systolic pressure/end-diastolic pressure (Pes/Ped), end-systolic volume/end-diastolic volume (Ves/Ved), relaxation time constant calculated by Weiss method (Tau) were all remarkably increased (P<0.05), but the peak rate of pressure rise (dP/dtmax), peak rate of pressure decline (-dP/dtmin), ejection fraction (EF) and stroke volume were obviously decreased in the HF group (P<0.05). Conclusion Pressure-overload results in left ventricular systolic and diastolic dysfunction, with partial hypertrophy at the same time. The P-V loop is a useful method for qualitative and quantitative assessment of left ventricular function in rats.
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