Protective effect of Xinfukang oral liquid on myocardial necrosis in rats following acute exhausted exercise
  
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DOI:10.3724/SP.J.1264.2013.00170
Key words:Xinfukang oral liquid  acute exhaustive exercise  cardiomyocyte necrosis
Author NameAffiliationE-mail
LIU Hai-Yan, WANG Chen-Yang, CUI Yu-Juan, ZHOU Yu-Wen, XU Peng, CAO Xue-Bin* (解放军第252医院心内科, 全军老年心血管病研究诊疗中心, 保定 071000) caoxb252@163.com 
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Abstract:
      Objective To observe cardiomyocyte necrosis in the rats induced by acute exhausted exercise and determine the protective effects of Xinfukang oral liquid (a traditional Chinese medicine) on the necrosis. Methods A total of 40 male Sprague-Dawley (SD) rats were randomly divided into 4 groups: control group, the low dose Xinfukang oral liquid [5ml/(kg·d)]+acute exhaustive group, the high dose Xinfukang oral liquid [10ml/(kg·d)]+acute exhaustive group, and acute exhaustive group (n=10 for each group). The Xinfukang oral liquid groups were treated with Xinfukang oral liquid at corresponding doses for 6 weeks by intragastrical administration before model induction, and the rats from the control group and acute exhaustive group were given normal saline instead of Xinfukang oral liquid. Acute exhausted exercise was carried out according to the classical Thomas’ induction, and then identified by the rats’ behaviors and signs. Myocardial necrosis was observed by transmission electron microscopy. DNA ladder were detected to observe myocardial cell DNA degradation. Western blotting was used to analyze the expression of total extracellular signal-regulated kinase (ERK) and phosphorylated ERK (p-ERK) in all groups. Results Transmission electron microscopy indicated that exhausting exercise group showed significantly more serious mitochondria swelling, obvious myocardial necrosis and DNA degradation. Compared with exhausted group, pretreatment of Xinfukang oral liquid resulted in attenuated injury in both myocardial cells and DNA integrity, and a increased ratio of p-ERK expression(P<0.05). Conclusion Pretreatment of Xinfukang oral liquid exerts a preventive effect on cardiomyocyte necrosis induced by acute exhaustive exercise.
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