The effect of androgen in vascular endothelial function of male rats under high-salt diet
  
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DOI:10.3724/SP.J.1264.2012.00241
Key words:androgen  hypertension  endothelin-1  nitric oxide  rat
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The effect of androgen in vascular endothelial function of male rats under high-salt diet Department of Geriatrics, Affiliated Sixth People's Hospital, Shanghai Jiaotong University, Shanghai 200233, China sjxt@yahoo.com.cn 
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Abstract:
      Objective To explore the effect of different levels of androgen in the vascular endothelial function of high-salt fed male rats. Methods Thirty-two Wistar rats were randomly assigned to 4 groups, sham-operation (Sham) group, castration (Cas) group, testosterone supplement after castration (TC) group, and flutamide-treated testosterone supplemented castrated (FTC) group, with eight rats in each. All animals were fed high-salt diet (8% NaCl) for 8 weeks. Animals in Sham group received sham operation, while those in Cas, TC and FTC group received surgical castration. Subcutaneous injection of testosterone propionate (TP) 2mg/(kg?2d) was performed in TC and FTC groups, and intragastric administration of flutamide 1mg/(kg? d) in FTC group continuously for 8 weeks. The blood of tail artery was measured at baseline, 4 and 8 weeks after experiment respectively. At 8 weeks after experiment, content of testosterone (T) and endothelin-1(ET-1) in serum were determined by enzyme-linked immunosorbent assay (ELISA), and colorimetric assay was used to detect the level of nitric oxide(NO) in plasma. Results (1) Compared with Sham group, blood pressure at 4 and 8 weeks declined significantly in Cas group and FTC group [Cas: (124.8±4.4) vs (135.9±3.7)mmHg, P<0.01; FTC:(127.7±3.0) vs (135.9±3.7)mmHg, P<0.01], while increased significantly (P<0.01) from baseline in Sham and TC groups. (2) Compared with Sham group, serum T content obviously decreased in Cas group [(0.26±0.02) vs (3.15±0.54)μg/L, P<0.01], and increased significantly in FTC groups [(5.47±1.29) vs (3.15±0.54)μg/L, P<0.01]. (3) Compared with Sham group, serum ET-1 content declined significantly in Cas group and FTC group [Cas: (0.77±0.23) vs (1.08±0.15)μg/L, P<0.01; FTC: (0.80±0.21) vs (1.08±0.15)μg/L, P<0.05], and had no significant difference with TC group (P>0.05). (4) NO content, compared with Sham group, increased significantly in Cas group and FTC group [Cas: (42.37±15.74) vs (17.20±7.32)mmol/L, P<0.01; FTC: (33.44±8.50) vs (17.20±7.32)mmol/L, P<0.01], and had no significant difference with TC group (P>0.05). Conclusion Under high-salt diet, change of androgen can influence vascular endothelial function of male rats.
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