Effect of salidroside on carbohydrate metabolism and serum levels of inflammatory cytokines in rats under negative psychological stress induced by experimental navigation and intensive exercise
  
View Full Text  View/Add Comment  Download reader
DOI:10.3724/SP.J.1264.2012.00240
Key words:rat  salidroside  carbohydrate metabolism  inflammatory cytokines
Author NameAffiliationE-mail
PENG Li, WANG Yangtian, WANG Qun, SUN Lingjun, WANG Jian, LI Jie Department of Endocrinology, Nanjing General Hospital, Nanjing Military Command, Nanjing 210002, China happypeng1193@yahoo. com. cn 
Hits: 1775
Download times: 1251
Abstract:
      Objective To determine the effects of salidroside on the carbohydrate metabolism and the serum levels of inflammatory cytokines in rats under negative psychological stress induced by experimental navigation and intensive exercise. Methods A total of 30 6-week Sprague-Dawley rats (SD rats) were randomly and equally allocated into 3 groups, quiescent control group (group A), training control group (group B) and salidroside treatment group (group C). Rats from group A received no stimulation, and administered a 7-day intragastrical injection of normal saline. Rats from group B underwent a 10-day run training with increasing load on treadmill and received 7-day intragastrical administration of normal saline. Group C were subjected to the same procedure of run training as group B, and received 7-day intragastrical administration of 0.4g/kg salidroside. Then, all the rats underwent experimental navigation and intensive exercise. Blood samples were immediately obtained for detection of blood glucose and the levels of inflammatory response-related cytokines, high sensitive-C reactive protein (hs-CRP), tumor necrosis factor α (TNF-α), interleukin 6(IL-6), and endothelin 1 (ET-1) by ELISA. Results As compared with group A, the levels of blood glucose, hs-CRP, TNF-α, IL-6, ET-1 was significantly increased in group B (P<0.05), while, the levels of blood glucose was significantly increased (P<0.05), and those of hs-CRP, IL-6, and ET-1 were significantly decreased in group C (P<0.05). The levels of hs-CRP, TNF-α, IL-6, ET-1 in group C were also significantly decreased than in group B (P<0.05). The level of blood glucose in group C showed a decreasing tendency. Conclusion Negative psychological stress and intensive exercise lead to disorders of glucose metabolism and increase the production of inflammatory cytokines in rats. Salidroside therapy reduces the levels of blood glucose and inflammatory cytokines in rats under stress, and thus improve glucose metabolism and enhances anti-stress capacity.
Close