Objective To study the senescence of glycometabolism(GM) with reduced hepatic clearance of insulin. Methods Totally 383 patients admitted in Chinese PLA 458th Hospital between March 2007 and October 2009 were recruited in the study, including 383 fasting cases and 87 oral glucose tolerance test(OGTT) cases. According to method of two factorial experiment, fasting patients were divided into type 2 diabetes(T2D), pre-diabetes(PD) and euglycemia group by GM; aged, middle and young group by age. Meanwhile, OGTT patients were divided into T2D and non-diabetes group by GM; aged and non-aged group by age. Biochemical assays including plasma glucose(PG), insulin(Ins) and C-peptide(C) were carried out for all patients. The quantitative data of assays were analyzed by SPSS13.0 software with ANOVA or S-N-K. Results In fasting state, patients of T2D group had highest level of plasma glucose, and PD group had the highest Ins and C-peptide levels (P＜0.05); middle age group had highest PG and C-peptide levels (P＜0.05), and the highest Ins was found in aged group; there was interaction of assays between GM and age (P＜0.05). With OGTT stimulation, early phase secretion of Ins was deficient in T2D group(P＜0.01); PG in aged group was lower than that in non-aged group, but Ins and C-peptide in aged group exceeded that in non-aged group 1-3 h after OGTT(P＜0.05). Conclusion There is senescence on GM: fasting Ins increases with age, and there is interaction between GM and senescence. More Ins released in blood is observed due to the reduced hepatic clearance in aged patients.